Molecular modeling and mechanism of action of human decay-accelerating factor

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Molecular and functional analysis of mouse decay accelerating factor (CD55).

Molecular cloning of mouse decay accelerating factor (DAF; CD55) predicted two forms of the molecule, one transmembrane (TM) and the other glycosylphosphatidylinositol (GPI)-anchored; these are encoded by separate genes termed Daf-GPI and Daf-TM. In the present study several additional isoforms of mouse DAF, generated by alternative splicing from these genes, are described. Northern-blot analys...

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The Expression and Action of Decay-Accelerating Factor (CD55) in Human Malignancies and Cancer Therapy

Decay-accelerating factor (DAF, CD55) is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. Here DAF seems to exert different functions beyond its immunological role such as e.g. promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis,...

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Molecular cloning of murine decay accelerating factor by immunoscreening.

Although the cDNA of human decay accelerating factor (DAF) which restricts complement activation on homologous cell membranes was cloned in 1987, all trials to detect the cDNA of mouse DAF by cross-hybridization were unsuccessful. However, by immunoscreening with a rabbit antiserum against purified mouse DAF, we successfully cloned the cDNA. It contains four typical short consensus repeats (SCR...

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ژورنال

عنوان ژورنال: "Protein Engineering, Design and Selection"

سال: 1996

ISSN: 1741-0126,1741-0134

DOI: 10.1093/protein/9.12.1143